| Cell Line Name |
SHEF-7 |
| Cell Line Status |
Currently available |
| Cell Type |
Human Embryonic |
| Grade |
Research |
| Source |
Human embryo / blastocyst |
| Source type |
Cryopreserved |
| Consent Status |
Donor consent was reviewed by the UK Steering Committee and was
found to be consistent with the Steering Committee Code of Practice
for the Use of Human Stem Cell Lines. |
| Date cell line derived |
22 December 2005 |
| Ethical Statement |
In the opinion of the Steering Committee for the UK Stem Cell
Bank and the use of Human Stem Cell Lines, the derivation of the
stem cell line is consistent with UK ethical guidelines |
| Depositor |
Pfizer |
| Depositing Centre |
Pfizer |
| Country of Origin |
United Kingdom |
| MUL Contact Details |
Timothy Allsopp [Timothy.Allsopp@pfizer.com] |
| Journal Reference |
NA |
| SCSC No. |
SCSC-06-30 |
| UKSCB Accession No. |
R-07-008 |
| Distribution Bank No. |
D01 |
| Passage No. |
p21 |
| Feeder Cell Type |
Mouse embryo fibroblast |
| Basel Medium |
KO-DMEM |
| Protein Source |
KO-SR |
| Growth Factors |
bFGF |
| Incubation Conditions |
37°C, 5% CO2, >95% RH, Subculture: manual
cutting |
|
Culture Morphology |
Embryonic stem cell colonial morphology |
| Preservation Method |
Frozen /DMSO |
| Viability |
<50% |
| DNA Profile |
Human |
| Karyotype |
46,XX |
| Characterisation Panel |
Confirmed SSEA-1 Low; Confirmed Positive for SSEA-3/SSEA-4,
TRA-1-60, TRA-1-81, TRA-1-85, TRA-2-54, OCT3/4; Awaiting
Confirmation SOX 2 |
| Sterility Test |
No contamination detected by EU Pharmocopeal Sterility
Test |
| Mycoplasm Screen |
Mycoplasma not detected by Mycoplasma PCR or Culture |
| Viral Screen |
Human Virus Panel (HIV, HTLV, HBV, HCV, EBV, CMV) not detected
by PCR |